Qing Sun
Address: 150 Academy Street, University of Delaware, Newark, DE 19716
Email: sunqing@udel.edu Phone: 951-333-7856
Education
University of Delaware Ph. D. candidate of Chemical and Biomolecular Engineering 2015.05(expected)
GPA: 3.94/4.0
Tsinghua University Master of Environmental Engineering 2009.06
GPA: 87.5/100
Wuhan University Bachelor of Environmental Engineering 2007.07
GPA: 89.7/100 Rank: 1/50
Bachelor of Biological Science
GPA: 82.3/100
Experiences
Graduate 08/2013 - present
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University of Delaware
Advisor: Dr. Wilfred Chen
esearch Assistant Post-translational modifications of protein-nanocages as a universal biosensor platform
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Successfully assembled protein nanocage based biosensors for thrombin and cancer marker detection.
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Overcame limitations of conventional bio-nanoparticle surface decoration strategies by using bio-friendly post-translational modification techniques.
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Achieved signal amplification by immobilizing multiple copies of sensor components on the same protein nanocage.
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Demonstrated the modularity of input and output domains to increase the universality of the platform for multiple diagnostic and therapeutic applications.
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Immobilized elastin-like polypeptide (ELP) on protein nanocages as purification tags to enable chromatography-free purification of assembled nanocages.
Graduate 12/2009 – 09/2013
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University of Delaware
University of California, Riverside
Advisor: Dr. Wilfred Chen
esearch Assistant Artificial cellulosomes construction on DNA template for efficient cellulose hydrolysis
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Successfully utilized ease to synthesize DNA scaffolds as templates for artificial cellulosomes assembly through Zinc Finger Protein (ZFP) guided immobilization.
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Elastin-like polypeptide was used as purification tag as well as a linker for ZFP and cellulases.
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Observed enhancements in cellulose hydrolysis comparable with already existing protein scaffolds based artificial cellulosomes.
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Targeting higher order-structure cellulosomes with DNA nanotechnology in anticipation of higher cellulose hydrolysis efficiency.
Graduate 11/2009 -12/2010
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Tsinghua University
Professor Zhenyu Li
esearch Assistant Low-cost defluoridation adsorbents development for drinking water safety
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Focused on developing Magnesium Oxide as defluoridation adsorbents using microwave processing followed by characterization.
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University of Delaware
Wilfred Chen lab
ab manager 09/2012 -10/2014
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Supervising Chen lab with up to 10 graduate students and 2 postdoctoral researchers.
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Ensuring resource planning, equipment functions, purchasing and maintenances of the whole lab to provide suitable working environment.
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Coordinated with the UDel Environmental & Health Safety Department to identify and address potential safety issues.
Skills
Molecular biology: Microbial protein expression and optimization; Protein purification (chromatographic and non-chromatographic); PCR; Plasmid extraction and transformation; DNA recombination; SDS-PAGE; Native PAGE gel; Western Blot; Immunofluorescence; ELISA; Protein-protein/molecular interactions; UV-VIS spectroscopy; Enzyme engineering Cell culture: Mammalian cell culture; Cell passaging; Cell fixation; Targeted drug delivery; Cell imaging
Native in Chinese, Full professional proficiency in English
Publications and Presentations
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Qing Sun and Wilfred Chen. Targeted drug delivery by tetrafunctional protein nanocage assembled through post-translational modification. In preparation.
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Qing Sun, Qi Chen, Daniel Blackstock and Wilfred Chen. Protein nanocage serving as a universal platform for cancer detection. In preparation.
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Qing Sun, Bhawna Madan, Shen-Long Tsai and Wilfred Chen. Creation of artificial cellulosome on DNA scaffolds by zinc finger protein-guided assembly for efficient cellulose hydrolysis. Chemical Communications, 2014, 50: 1423-1425.
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Qi Chen, Qing Sun, Szu-Wen Wang, Eric T. Boder and Wilfred Chen. Thermally-responsive and biocatalytic protein nanocages by sortase-mediated stepwise modular display. In preparation.
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Daniel Blackstock, Qing Sun, and Wilfred Chen. Fluorescent protein-based molecular beacons by zinc finger protein-guided assembly. Biotechnology and Bioengineering. Accepted for publication.
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Ka-Hei Siu*, Rebecca Chen*, Qing Sun*, Long Chen*, Shen-Long Tsai* and Wilfred Chen. Molecular scaffolds for pathway enhancement. Current Opinion in Biotechnology. In preparation. (*equal contribution)
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Miso Park, Qing Sun and Wilfred Chen. Positional assembly of enzymes on bacterial outer membrane vesicles for cascade reactions. PLoS ONE 9(5): e97103. doi:10.1371/journal.pone.0097103.
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Rebecca Chen*, Qi Chen*, Heejae Kim*, Ka-Hei Siu*, Qing Sun*, Shen-Long Tsai* and Wilfred Chen. Biomolecular Scaffolds for Enhanced Signaling and Catalytic Efficiency. Current Opinion in Biotechnology. 2014, 28:59–68. (*equal contribution)
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Daniel Blackstock*, Miso Park*, Qing Sun*, Shen-Long Tsai* and Wilfred Chen. Engineering protein modules for diagnostic applications. Current Opinion in Chemical Engineering, 2013, 2: 416-424. (*equal contribution)
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Heejae Kim*, Qing Sun*, Fang Liu*, Shen-Long Tsai* and Wilfred Chen. Biologically assembled nanobiocatalysts. Topics in Catalysis, 2012, 55: 1138-1145. (*equal contribution)
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Qing Sun, Shen-Long Tsai, Bhawna Madan and Wilfred Chen. Functional self-assembly of artificial cellulosomes for efficient cellulose hydrolysis. AIChE, October 2012, Pittsburgh, Pennsylvania.
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Qing Sun, Qi Chen, Eric T. Boder, Szu-Wen Wang and Wilfred Chen. Post-translational modification of artificial cellulosomes for efficient cellulose hydrolysis. AIChE, November 2013, San Francisco, California.
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Qing Sun, Qi Chen, Szu-Wen Wang and Wilfred Chen. Post-translational modification of protein nanocages as a universal biosensor platform. ACS, March 2014, Dallas, Texas. (Poster)
Teaching Experiences
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Teaching Assistant. University of California, Riverside.
CHE 160C Chemical Engineering Lab 03/2010-06/2012
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Guide for the Sino-Italian Tsinghua Environmental and Energy Efficient Building, China. 07/2008~09/2008
Selected Honors and Awards
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Graduate fellowship, University of California, Riverside USA 09/2009
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Third Prize of Summer Employment Practice, Tsinghua University China 08/2008
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Second Prize at the 6th Challenge Cup, National and Hubei Provincial Student Extracurricular
Academic Science and Technology Work Competition China 06/2007
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