DuPont Qualicon bax® System

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DuPont Qualicon BAX

®

 

System 

PCR ASSAY FOR GENUS 

LISTERIA 

24E 

PRODUCT DESCRIPTION  

 

 

Listeria species are indicator organisms whose detection can indicate that pathogenic Listeria monocytogenes is 

present. Listeria monocytogenes can cause serious illness in persons with heightened susceptibility, especially 

pregnant women and immunocompromised persons. Traditional culture methods can take four days or more to get 

results, and many rapid methods require a two-stage, 48-hour enrichment. With this BAX

®

 system assay, however, 

accurate and reliable results are available the next day with a single-step, 24-hour enrichment.

 

 

 

 

 

BAX

®

 System PCR Assay  

Genus Listeria 24E 

 

Part # QB8135C 

 

96 tests per kit 

 

PCR tubes with tablets, optical caps, 

lysing agents, protease, lysis buffer 

 

Store at 2-8°C 

 

Stable to expiration date on label 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Benefits 

•

 

Speed – Single-stage enrichment complete in only 24 hours  

•

 

Accuracy – Automated DNA-based analysis  

•

 

Exceptional sensitivity – Detects 10

4

 cfu/mL  

•

 

Ease of use – Tableted reagents reduce operator error 

•

 

Closed-cap system avoids amplicon contamination in the lab 

•

 

LIMS-compatible electronic data for easy storage, sharing and retrieval 

•

 

Designed for efficient workflow and reliable results

 

 

Features 

•

 

PCR processing completes in 3.5 hours 

•

 

Can be run in mixed batches with other BAX® system standard assays  

•

 

Validated on meat, seafood, dairy, vegetable and environmental samples

•

 

Detects all species of Listeria except some strains of L. grayi 

•

 100% 

exclusivity

 

 

•

 

Next-day results with 24 LEB media (CM1107/SR0243E) 

 

Approvals 

•

 

AFNOR validated to ISO 16140; Certificate number QUA 18/06 – 07/08 

    

   

 

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. 

 

 

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Copyright © 2007 DuPont. All rights reserved. The DuPont oval logo, DuPont™, The Miracles of Science™ and BAX® are trademarks or registered trademarks of DuPont or its affiliates. BAX® System Q7 

instruments are sold under licensing arrangement with Applied Biosystems for food testing. Use for research and development, quality assurance and quality control testing under supervision of technically 

qualified persons. Not approved for clinical use. Please read the limitation of warranty and liability before use. 27A-031.1-0608 

BAX® System PCR Assay for Genus 

Listeria 24E 

Page 2

 

Sample preparation  

 

 

 

Enrichment:  Homogenise samples (1:10) in prepared 24 LEB 

(CM1107/SR0243E) using filterless stomacher bags.  

Incubate 24 hours at 37°C. 

 

 

BAX® system protocol 

 

10:00

 Create rack file and 

warm up cycler. 

10:05

 Dilute Lysing agent 1 

with 1.8 mL sterile water, and 

combine in 4:1 ratios with 

Lysing agent 2.  

10:10

 Transfer 50 µL of 

combined agents to cluster 

tubes, then add 0.5 mL 

enriched sample and incubate 

for 30 minutes at 37°C. 

 

 

 

 

 

10:50

 Mix protease with 

lysis buffer and transfer  

200 µL of lysis reagent to 

new cluster tubes. 

10:55

 Transfer 5 µL of 

lysate to cluster tubes. 

11:00

 

Heat cluster tubes for 

30 minutes at 55°C, then 10 

minutes at 95°C. Cool for 5 

minutes in lysis cooling block. 

 

 

 

 

 

 

11:45

 

Hydrate PCR tablets in 

second cooling block with  

50 µL of chilled lysate.

 

11:50

 Place sealed PCR 

tubes in cycler and run 

program.

 

3:20

 

Review results.

 

 

 

 

 

 

Oxoid 

Wade Road 

Basingstoke 

Hampshire, RG24 8PW, UK 

Tel: [phone] 

Fax: [phone] 

Email: 

[e-mail] 

 

 

 

 

 

 

Folio No.  1249 08/08 

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Document Outline

• Benefits
• Features
• Approvals
• BAX® System PCR Assay for Genus Listeria 24E Page 2
• Sample preparation 
• BAX® system protocol