Gencrispr™ Custom Cell Line Development Service sc1755

GenCRISPR™ Custom Cell Line Development Service

SC1755 (EZ Knock-out Package)

Instructions

Please complete and email this form to [e-mail] 2. Our Account Manager will contact you with a quote.

Customer Information

If you have registered an account with GenScript, you can identify yourself by giving us your name and email address or Account No.

Name:	     
Account No.:	     
Phone:	     
Organization:	     
Shipping Address: (Necessary to determine shipping cost)	     
Email Address:	     

Genomic Editing

Which of following service are you looking for?  Single gene knockout in a cell line  Multiplex gene knockout in a cell line. Specify gene number:_____________

Name of Target Gene: NCBI Accession Number (Gene ID):

What is type of the area for targeting?  Protein coding  MicRNA  lncRNA  Promoter  Transcription factor binding site  Others. Specify__________

Do you need GenScript to design gRNAs:  No. Please provide gRNA sequences to be used:_______________________________________________________________  Yes. Please specify the gene products (e.g. NCBI accession ID NM or NP) or sequence to be disrupted:____________________ _________________________________________________________________________________________________________ _________________________________________________________________________________________________________

Does KO of the target gene inhibit cell proliferation?  Yes  No  Not sure If yes or no, please provide an evidence to support your choice:____________________________________________________

Does KO of the target gene inhibit cell survival?  Yes  No  Not sure If yes or no, please provide an evidence to support your choice:____________________________________________________

Host Cell Line

Name of Host Cell Line: A375 CHO-K1 HCT116 HEK293 HEK293T Hela HEP-2 U2OS

How many copies of the target gene exist in the cell line?  One copy  Two copies  More. Specify copy number: _________  Not sure Please provide an evidence to support your choice:____________________________________________________

Do you need GenScript to follow any special cell culture routine?  Yes, see below  No Please provide the protocol with information about the cell line and any special growth characteristics or requirements: __________________________________________________________________________________________

Requirements for Customized Engineered Cell Line

Which of following genotype of knockout cell line do you want: Single allele Two alleles Multiple alleles (indicate number)______ All alleles (number unknown)

How many knockout clones do you want (Extra fees may be charged):  One clone  Two clones  Three clones  More clones, specify the amount:_________________

Please indicate the preferred type of optional analysis to characterize transgenic cell line (Extra fees may be charged):  Growth curve  Western blot (validated antibodies to be provided by customer)  Off-target analysis  Reverse-transcribe PCR  qPCR analysis

Could you please describe what your application is with the KO cell line?  Gene function analysis  Assay development  Drug screening Bioproduction  Other. Please indicate your specific application and requirements:_________________________________

Comments:      

Project Information

Is this project for grant application purpose?  Yes  No

When will the project start?  Immediately  Within one month  Within three months  Half a year later