Supplementary Figure 1 Cell death seems to be unaffected in aurA mutant brains

Supplementary Figure 1 Cell death seems to be unaffected in aurA mutant brains. Both wild-type (A) and aurA¹⁷⁹⁶¹ mutant brains (A’) display similar numbers of Caspase-3-expressing cells. DNA is in blue.
Supplementary Figure 2 aurA display overgrowth phenotype in MACRM clones. Single NB MARCM clones were marked by CD8 (A’, B’, C’, D’, E’, F’). In aurA⁸⁸³⁹ clones, multiple cells expresses Mira (B), Wor (D), and CycE (F) compared to wild-type clones in which only one cell (NB) expresses Mira (A), Wor (C), and CycE (E).
Supplementary Figure 3 Numb is a tumor suppressor in larval brains. Single NB MARCM clones were marked by CD8 in A’, B’, C’, D’. In numb¹⁵ clones, most of cells expresses Mira (B) but very few cells expresses Pros (D) in nucleus compared to wild-type clones in which a single NB expresses Mira (A), and most of cells express nuclear Pros (C).
Supplementary Figure 4 Notch is necessary and sufficient for NB proliferation. A-D’, overexpression of N^act in the clone induces NB overproliferation. Clones were marked by -Gal in A’, B’, C’, D’. In N^act clones, most of cells express Dpn (B) and Elav is almost absent (D) compared to control clones in which a single NB expresses Dpn (A) and most of cells express Elav (C). E-G’, Overexpression of N^act results in upregulation of CycE and dMyc. Overexpression of N^act by a wor-Gal4 driver controlled by a tubulin-Gal80^ts in the whole larval brain results in NB overproliferation when shifted to 29C after larval hatching (E’). Mutants have supernumerary number of NBs marked by Dpn (E’) and upregulation of CycE (F’) and dMyc (G’) compared to a control wor-Gal4;tubulin-Gal80^ts brain that expresses Dpn (E), CycE (F), and dMyc (G) normally. H, H’, the number of CycE-expressing cells in Notch^ts-1 larval central brains (H’) is decreased compared to wild-type (H).