The media we will be using in the Plant Microbiology Lab mg/L



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The media we will be using in the Plant Microbiology Lab
MG/L (Chilton 1974)

2.5 g Tryptone

1.25 g yeast extract

2.6 g NaCl

5 g mannitol

1.16 g sodium glutamate

0.25g KH2PO4

0.1 g MgSO4.7H2O

1 ug biotin

Water to 500 ml

pH to 7.0

For plates, add 7.5 g bacto-agar


Nitrogen-Limited Medium: Combined Carbon Medium (RJ Rennie 1981)

Solution 1:

Sucrose 5 g/L

Mannitol 5 g/L

Sodium Lactate (ml, 60%, v/v) 0.5 ml/L

K2HPO4 0.80 g/L

KH2PO4 0.20 g/L

NaCl 0.10 g/L

Na2Mo04.2H20 25.0 mg/L

Na2FeEDTA 28.0 mg/L

Yeast Extract 100mg/L

Distilled Water 900ml

(If agar, use 10-15 g Phytagar/L)



Solution 2:

MgSO4. 7 H20 0.20 g/L

CaCl2 0.06 g/L

Distilled water 100 ml



Autoclave Solution 1 and 2 separately, cool to 50C and mix.


10X Nitrogen Free Medium (Qubit Systems recipe)- 1 liter

5 ml Stock 1 (0.514M KH2PO4)

5 ml Stock 2 (0.114M K2HPO4)

5 ml Stock 3 (1.004M K2SO4)

5 ml Stock 4 (0.486M MgSO4*7H2O)

5 ml Stock 5 (0.492M MgCl2*6H2O)

5 ml Stock 6 (1.496M CaCl2*H2O)

5 ml Stock 7 (0.020M MnSO4*H2O)

5 ml Stock 8 (0.002M CuSO4*5H2O)

5 ml Stock 9 (0.002M ZnSO4*7H2O)

5 ml Stock 10 (0.062 M H3BO3)

5 ml Stock 11 (0.001 M Na2MoO4*2H2O)

5 ml Stock 12 (0.0004M CoSO4*6.5H2O)

5 ml Stock 13 (0.076M Fe Sequestrine)=3.5 g FeSequestrine/100ml



Water to 1 L; Pour into a large beaker; pH to about 6.8; Filter-sterilize

Grow plants in 1X of this solution (dilute 50 ml in 500 ml water)

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