1/2 x Murashige & Skoog (MS) medium (1L)
MS salts with macro- and micronutrients (Caisson Laboratories, Inc. Cat # 2.165 g
MSP0501) (stored at 4C)
Sucrose 10 g
dH2O to 1L
Bacto agar 8g
Autoclave at 121 C for 30 min.
About 30 ml/plate
To make selective plates, let medium cool down to about 60C then add antibiotics. Mix well before pour plates.
(Note: some people also use 2 or 3% of sucrose. If you are not going to work on carbon metabolism or certain phtohormones, this is not very important.)