The media we will be using in the Plant Microbiology Lab
MG/L (Chilton 1974)
2.5 g Tryptone
1.25 g yeast extract
2.6 g NaCl
5 g mannitol
1.16 g sodium glutamate
0.25g KH2PO4
0.1 g MgSO4.7H2O
1 ug biotin
Water to 500 ml
pH to 7.0
For plates, add 7.5 g bacto-agar
Nitrogen-Limited Medium: Combined Carbon Medium (RJ Rennie 1981)
Solution 1:
Sucrose 5 g/L
Mannitol 5 g/L
Sodium Lactate (ml, 60%, v/v) 0.5 ml/L
K2HPO4 0.80 g/L
KH2PO4 0.20 g/L
NaCl 0.10 g/L
Na2Mo04.2H20 25.0 mg/L
Na2FeEDTA 28.0 mg/L
Yeast Extract 100mg/L
Distilled Water 900ml
(If agar, use 10-15 g Phytagar/L)
Solution 2:
MgSO4. 7 H20 0.20 g/L
CaCl2 0.06 g/L
Distilled water 100 ml
Autoclave Solution 1 and 2 separately, cool to 50C and mix.
10X Nitrogen Free Medium (Qubit Systems recipe)- 1 liter
5 ml Stock 1 (0.514M KH2PO4)
5 ml Stock 2 (0.114M K2HPO4)
5 ml Stock 3 (1.004M K2SO4)
5 ml Stock 4 (0.486M MgSO4*7H2O)
5 ml Stock 5 (0.492M MgCl2*6H2O)
5 ml Stock 6 (1.496M CaCl2*H2O)
5 ml Stock 7 (0.020M MnSO4*H2O)
5 ml Stock 8 (0.002M CuSO4*5H2O)
5 ml Stock 9 (0.002M ZnSO4*7H2O)
5 ml Stock 10 (0.062 M H3BO3)
5 ml Stock 11 (0.001 M Na2MoO4*2H2O)
5 ml Stock 12 (0.0004M CoSO4*6.5H2O)
5 ml Stock 13 (0.076M Fe Sequestrine)=3.5 g FeSequestrine/100ml
Water to 1 L; Pour into a large beaker; pH to about 6.8; Filter-sterilize
Grow plants in 1X of this solution (dilute 50 ml in 500 ml water)
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