BION-M2:
Scientific
program
2
Space vehicle
Years
2016
2017 2018 2019 2020 2021 2022 2023 2024 2025
«BION-M» №2 «BION-М» №3
Flight – 30 days
Н=800-1000 km Н=550 km
«Vozvrat-MKA» №1
Flight – 30-45 days
Н=1000-200000 km
- Preparation
- Lunch
- Landing
Space Biology, Medicine and Biotechnology Research:
Russian Unmanned Spaceflights Scheduled for 2016 - 2025
- Flight
№2
№3
№1
BION-M No. 2 Project
Beginning of funding – 2015
Planned launch date– 2022
Flight duration– 30 days
Orbital height– 800-1000 km
The hardware will be analogous to BION-M1, but it will be modified based on the
results of flight tests.
Bioobjects –C57BL/6 mice, insects, plants, cell cultures, microorganisms
Main task:
Comprehensive study of combined biological impact of increased space radiation
levels and weightlessness on organism and its separate functional systems at te
cellular and molecular levels.
37 Technical Specifications for space experiments aboard BION-M2 from 23 RAS institutes,
universities, and other research institutions submitted to the Interagency committee:
1.
Institute of Biomedical Problems of RAS
2.
Federal Research Center – Institute of Cytology of RAS
3.
FSBRI – Institute of Fundamental Problems of Biology of RAS
4.
FSBRI – Institute of Physicochemical and Biological Problems of Soil Science of RAS
5.
FSBRI – Institute of Systems of Image Processing of RAS
6.
FSBRI – Institute of Crystallography of RAS
7.
FSBRI – Institute of Solid State Physics of RAS
8.
Orenburg Research Center of Ural Branch of RAS
9.
FSUE Special Design Bureau of the Institute of Radiotechnique and electronics of RAS
10. Biological Department of Lomonosov Moscow State University
11. State Research Institute of Genetics and Selection of Industrial Microorganisms
12. The All-Russian Research Institute for Optical and Physical Measurements Federal State Unitary
Enterprise
13. Space Rocket Center “Progress”
14. Laboratory of Radiation Biology in Joint Institute for Nuclear Research ;
15. Tuscia University, Italy
16. Sapienza — Università di Roma, Italy
17. FSUE Branch - Center for Operation of Ground-based Space Infrastructure – Research Institute of
Launch Facility
18. FSBRI Perm State National Research University
19. Institute of Space Instrument Engineering of the Korolev’s Samara State Aerospace University
20. Samara State Medical University of Health Ministry
21. FSUE “Samara Regional Center of Planned Parenthood and Reproduction”
22. Samara State University
23. FSBRI – Samara Research Institute of Agriculture
After review of the TS-SE:
27 + 3*
)
experiments and investigations have been included into the BION-M2 flight
tests program,
6 experiments (3 + 3*
)
) and investigations have been included conditionally, and
4 experiments have been rejected.
The experiments and investigations during flight tests of BION-M No. 2 SV will be conducted
in the following fields:
1.
Experiments with animals - 3
2.
Experiments with animals cell cultures
3.
Experiments with plants, plant cells cultures, seeds, and algae – 6
4.
Experiments with microorganisms – 4
5.
Exobiological and astrobiological experiments – 4+1*
6.
Radiobiological experiments and radiation studies – 1
7.
Biotechnological experiments – 2
8.
Investigations and experiments on space materials science 1+1*
9.
Physical and technical experiments – 4+1*
10. Educational experiments – the program will be formed based on the applications from
educational institutions at the finals stages of SV preparation.
Note: *
)
conditionally included experiments which have priority since SH (Scientific Hardware) for them is included
into the preliminary list of SH, approved by the RAS Space Board on 03.12.2014
Experiments with animals
1. SE «Study of С57BL/6 mice during flight of the BION-M No. 2 space vehicle”,
Code «MLZh-02».
Principle Investigator: Doctor of Biological Sciences, Prof. Olga Vinogradova.
Leading institution: Institute of Biomedical Problems of RAS
Aim: Comprehensive study of combined biological effects of increased space radiation
levels and weightlessness on the organism and its separate functional systems at the cellular
and molecular levels.
2. SE «Study of С57BL/6 mice during flight of the BION-M No. 2 space vehicle”,
Code «MLZh-02-02».
Principle Investigator: Doctor of Biological Sciences, Prof. Olga Vinogradova.
Leading institution: Institute of Biomedical Problems of RAS
Aim: Comprehensive study of combined biological effects of increased space radiation
levels and weightlessness on the organism and its separate functional systems at cell and
molecular levels at changed conditions of animals management with regard to the tasks to
be solved during BION-M No. 3 project.
C57Bl/6 mice
(n=75)
Males
Females
Food type
Paste
68% water
(n=60)
Dry +
water
(n=15)
Hardware
Bios-02
Significantly
Modified
Bios-01
Bios-02-02
Re-designed
habitats suitable for
centrifugation
Areas of research:
• CNS & sensory system
• Muscles
• Bones
• Fluid-electrolyte
metabolism & excretion
• Cardiovascular system
• Gender differences
PREPARATION
FLIGHT/
EXPOSURE
POSTFLIGHT
INVESTIGATIONS
Group formation
Surgeries
Tests
Animal selection
Orbital flight
Metabolism, BP &
HR, behavior
monitoring
Animal in vivo
examinations and
tissue investigations
1 hr, 12 hr, 5 & 15
days after recovery
Group formation
Surgeries
Tests
Animal selection
Group formation
Surgeries
Tests
Animal selection
Simulated flight
Metabolism, BP &
HR, behavior
monitoring
Animal in vivo
examinations and
tissue investigations
1 hr, 12 hr, 5 & 15
days after recovery
Vivarium
Metabolism, BP &
HR, behavior
monitoring
Animal in vivo
examinations and
tissue investigations
1 hr, 12 hr, 5 & 15
days after recovery
FLIGHT EXPERIMENT
DELAYED CONTROL EXPERIMENT
VIVARIUM CONTROL
List of foreign participants of the BION-M2 project
submitted their proposals
Country
Institution
Financing
institution
USA
NASA
NASA
JSC NASA
ARC NASA
US universities (will be determined after selection
process organized by NASA till 30 September
2017)
Applications have been submitted by:
Berkeley University (California)
Washington University (Washington)
California University(San Francisco)
California University (San Diego)
Minnesota University (Minneapolis)
Stanford University (California)
Florida University
List of foreign participants of the BION-M2 project
submitted their proposals
France
CNES
Cannes University
Montpellier University
Lille University
Angers University
Marcel University
CNES
Italy
Genoa University
Rome University
Padua University
Bari University
ASI
Germany
Otto-von Guericke-University Magdeburg
DLR
Kazakhstan
Institute of Human and Animal Physiology
Ministry of
Education and
Science
Malaysia
Department of Technologies and Innovations of
Angkasa
Agensi Angkasa
Negara
China
Center of Space Life Science & Life Support
Technology, School of Biological Science and
Medical Engineering
Ministry of
Education and
Science
Country
Institution
Financing
institution
3. SE «Search of potential mechanosensors of Drosophila melanogaster cells
exposed to the conditions of BION-M No. 2 space flight, code «MSKD».
Principle Investigator :
Doctor of Physico-mathematical Sciences Irina Ogneva.
Leading Institution:
Institute of Biomedical Problems of RAS
Aim: search of candidates to the role of target proteins which are potential
mechanosensors in Drosophila melanogaster cells exposed to the conditions of
BION-M No. 2 space flight.
Experiments with animals
1. SE «Study of SF factors effect on morpho-functional state, proliferative and developmental potential of
hematopoietic stem cells», code – «MULTIINCUBATOR».
Principle Investigator : MD, Prof. I. Davydkin.
Leading institutions:
Samara State Medical University of Health Ministry;
FSUE “Samara Regional Center of Planned Parenthood and Reproduction”;
Samara State University
Aim: study of SF factors effects on morphological and phenotypical characteristics of hematopoietic
stem cells (HSC).
2. SE «Study of SF factors effect on transcriptome of human mesenchymal stromal cells”, code
«TRANSCRIPTOME MSK».
Principle Investigator : RAS Corresponding Member L. Buravkova.
Leading institutions:
Institute of Biomedical Problems of RAS
Samara State University
Purpose of the experiment – study of SF factors effects on transcriptional activity of human cultured
mesenchymal stromal cells (MSC)
Experiments with animals cell cultures
1. SE «Study of SF factors influence on higher plants which are used in medical and
pharmaceutical practice” (by the example of Calendula officinalis L. and Echinacea
Purpurea), code – « PHYTO-2».
Principle Investigator : Doctor of Pharmaceutical Sciences Prof. V. Kurkin.
Leading Institution:
Samara State Medical University of Health Ministry.
Aim:
assessment
of
the
effect
of
combination
of
SF
factors
on
anatomic-
morphofunctional parameters and chemical composition of medical plants in
ontogenesis (by the example of Calendula officinalis L. and Echinacea Purpurea).
2. SE «Study of the dynamics of phototropic reactions of higher plants bines, by the example
of Physcomitrella patens (Hedw.) B.S.G.moss, under the influence of light stimulus in
different areas of light spectrum in microgravity”, code - «GRAVISENSOR-3».
Principle Investigator : Doctor of Technical Sciences, Prof. Yury Berkovich.
Leading institutions:
Institute of Biomedical Problems of RAS;
Biological Department of Lomonosov Moscow State University
Aim: study of the dynamics of
phototropic reactions of Physcomitrella patens (Hedw.) B.S.G.
moss bines under the impact of light stimulus in different
areas of light spectrum in microgravity.
Experiments with plants, plant cell cultures, seeds,
and algae
3. SE “Study of SF factors effect on physiology, biochemistry, and genetics of plant cells and
tissues of food-significant agricultural plants” , code «GENETIKA-2».
Principle Investigator : Candidate of Agricultural Sciences A. Milyokhin
Leading institute:
FSBRI – Samara Research Institute of Agriculture
Aim: study of SF factors effects on physiology, biochemistry, and genetics of plant cells
and tissues of grain, grain legumes, and industrial crops for further creation of high-
producing genotypes and varieties resistant to unfavourable
abiotic and biotic
environmental factors for their use in agro-industrial sector of Russian Federation.
4. SE «Assessment of the SF factors influence on the seeds obtained from rare plants of natural
flora cultivated from the seeds exposed on board BION-M No. 1 and FOTON-M No. 4”–
«FLORA-BS2».
Principle Investigator : Doctor of biological sciences Prof. L. Kavelenova.
Leading Institute:
Samara State University
Aim: assessment of the influence of different combinations of SF factors on seeds
germinating ability, post-flight development of seedlings, and further ontogenesis phases
of first generation of rare plants of natural flora cultivated from the seeds exposed on
board BION-M No. 1 and FOTON-M No. 4, in order to reveal the effect of repeated SF
factors influence in several generations.
Experiments with plants, plant cell cultures, seeds,
and algae
5. SE «Study of space flight factors influence on genetic, morphobiological, and biochemical signs
and characteristics of cell culture of Rhodiola rosea L. rootstock”, code – “RHODIOLA”.
Principle Investigator: Candidate of Agricultural Sciences A. Milyokhin
Leading institutions :
FSBRI – Samara Research Institute of Agriculture;
Samara State Medical University of Health Ministry
Aim: Study of the spaceflight factors impact on the pattern and dynamics of resynthesis
substance accumulation by various strains of cellular biomass of roseroot (Rhodiola rosea L.)
rootstock that represent interest for pharmaceutical industry, and also to receive in cells
positive genetically fixed mutations and develop new effective genetically stable strains and
their further use in modern pharmaceutical drugs and nutritive bioactive additives for humans
and animals
6. SE “Study of peculiarities of the growth and genetic-morphological changes in algae cells in space
flight”, code “VOZROZHDENIYE”.
Principle Investigator: Doctor of Biological Sciences Prof. A. Kurakov
Leading institute:
Biological Department of Lomonosov Moscow State University
Aim: study of peculiarities of the growth and genetic-morphological changes in algae cells in
space flight conditions for the benefit of fundamental botanic, and creation of biosensors on the
basis of cells for control of ecological quality of aquatic habitats, also in space greenhouses.
Experiments with plants, plant cells cultures, seeds,
and algae
1. SE «Study of space flight factors influence on morphobiological characteristics and properties
of fungi of soil rhizosphere
Trichoderma viride and Trichoderma harzianum»,
code–
« BIOMICROBE».
Principle Investigator: Candidate of Agricultural Sciences A. Milyokhin
Leading institute:
FSBRI – Samara Research Institute of Agriculture.
Aim: study of SF factors influence on genetics, physiology, and morphology of fungi of soil
rhizosphere Trichoderma viride and Trichoderma harzianum and further creation on its
basis of highly productive biological products of new generation for the use in agricultural
sector of Russian Federation.
2. SE «Study of space flight factors influence on life activity of microorganisms of different
classes”, code «BIOCOSM».
Principle Investigator: Doctor of Medical Sciences Prof V. Ilyin
Leading institutions:
Institute of Biomedical Problems of RAS;
Biological Department of Lomonosov Moscow State University
Biological Department of Lomonosov Moscow State University
State Research Institute of Genetics and Selection of Industrial Microorganisms
Aim: comprehensive study of space flight factors influence on life activity and functional
characteristics of microorganisms of different classes for the benefit of fundamental science
and solution of applied problems in the field of biology, biotechnology, and medicine.
Experiments with microorganisms
3. SE «Study of space flight factors influence on interaction between microorganisms and non-
metallic materials», code: «VZAIMODEYSTVIYE».
Principle Investigator: Doctor of Biological Sciences N. Novikova
Leading institute:
Institute of Biomedical Problems of RAS;
Aim: comprehensive study of SF factors influence on the interaction between bacteria/fungi
and polymeric substrate.
4. SE «Study of the effects of SF factors influence on bioprofiles of enterobacteria and
staphylococci», code: «ENDOFLORA-2».0
Principle Investigator: Doctor of Medical Sciences Prof. A. Zhestkov
Leading institutions:
Samara State Medical University of Health Ministry
Samara State Medical University of Health Ministry
Samara State University
Aim: study of SF factors influence on bioprofiles of enterobacteria and staphylococci,
typical representatives of human microbiota, also in the conditions of simultaneous
cultivation.
Experiments with microorganisms
1. SE «Study of a possibility of bioobjects surviving in mineral section of imitator of meteorite while it is passing
dense
atmosphere with accompanying heating and melting», code « METEORITE».
Principle Investigator: Doctor of Medical Sciences Prof V. Ilyin
Leading institutions:
Institute of Biomedical Problems of RAS;
Biological Department of Lomonosov Moscow State University.
Purpose of the experiment – study of a possibility of surviving of bioobjects, belonging to different taxonomic groups,
in mineral section of meteorite while it is passing dense atmosphere with accompanying heating and melting.
2. SE «Study of combined impacts of SF factors and minerals of exterrestrial origin on the synthesis and resistance of
bioorganic molecules”, code “EXOBIOLOGIYA”
Principle Investigator: Doctor of Biological Sciences E. Kuzicheva
Leading Institute:
Federal Research Center – Institute of Cytology of RAS
Purpose of the experiment – study of the effects of combined impact of SF factors and mineral bases on the process of
abiogenic synthesis of bioorganic compounds - polypeptides and nucleotides.
3. SE «Reaction of permafrost microorganisms to the impact of SF factors”, code “EXOBIOFROST”.
Principle Investigator Candidate of Biomedical Sciences E. Rivkina
Leading institute:
FSBRI – Institute of Physicochemical and Biological Problems of Soil Science of RAS
Purpose of the experiment – Study of BION-M No. 2 SF factors impact on physiological characteristics and genetic
changes in DNA structure of pure bacterial cultures and protists extracted from natural permafrost samples
(multiple-aged permafrost sediments of Arctic and Antarctica)
Exobiological experiments
4. SE «Study of SF factors impact on bioprofiles of lichen and micromycete”, code
“EXOMIKOLOGIYA”.
Principle Investigator: Doctor of Biological Sciences A. Kurakov
Leading institute:
Biological department of Lomonosov Moscow State University
Purpose of the experiment - Study of BION – M No. 2 SF factors on morphological and
physiological characteristics of intact blastema, on morphological, physiological, and
genetic changes in pure micromycete cultures, on composition of soil micromycete
communities.
5*. SE «Study of a possibility of synthesis of prebiotic compounds from mixture
“formamidemete + meteoritic material” under the open space conditions during BION-M No.
2 space flight”, code “ASTROBIO”.
PIs: RAS academician A. Rozanov, Prof. R. Saladino, Prof. E. Di Mauro
Leading institutions:
Laboratory of Radiation Biology in Joint Institute for Nuclear Research;
Tuscia University, Italy
Sapienza — Università di Roma, Italy
Purpose of the experiment – obtaining of new data about consequence of processes which
can lead to the formation of full chemically active prebiotic system.
Exobiological experiments
1. SE «Study of SF factors impacts on the processes of microbial utilization of organic substrates”, code
«BIOUTILIZATSIYA».
Principle Investigator: Doctor of Medical Sciences Prof V. Ilyin
Leading institution:
Institute of Biomedical Problems of RAS.
Purpose of the experiment – study of SF factors influence on biological activity and fermentative
characteristics of cellulolitic microorganisms of different taxonomic groups with further assessment of
efficiency and perspective of the use of bacteria-niodestructures for degradation of waste containing
cellulose.
2. SE «Study of the SF factors impact on electric-chemical characteristics of microbial fuel element», code
«MTE-2».
Principle Investigator: Candidate of Chemical Sciences I. Smirnov
Leading institution:
Institute of Biomedical Problems of RAS.
Purpose of the experiment – study of the effect of the factors of space flight in high orbit SV on
electrical-chemical characteristics of newly developed scientific hardware – microbial fuel element with
electrodes based on capillary-porous materials, and on the changes in specific and quantitative
composition of microbial association
Biotechnological experiments
1. SE «Growing of protein crystals by the methods of liquid and gas diffusion on board BION-M No. 2 SV
inside BELKA-2 scientific hardware”, code “BELKA-2”.
Principle Investigator: Doctor of Physico-mathematical Sciences A. Voloshin
Leading institutions:
FSBRI – Institute of Crystallography of RAS
National Research Center «Kurchatov’s Institute»;
FSUE Branch - Center for Operation of Ground-based Space Infrastructure – Research Institute of Launch
Facility
Purposes of the experiment:
• To study the SF conditions impact on the process of protein crystals growing by means of different
methods;
• To grow in SF conditions the crystals of high structural perfection of practically useful protein
compounds, which crystallization is not possible or hardly possible in laboratory conditions on the
Earth.
1*. SE
«Growing of biocrystals with active control of crystallization process, and determination of
incubation period crystal nucleation in space flight conditions on board BION-M No. 2 SV, code
«CRYSTAL-M».
Principle Investigator: Doctor of Technical Sciences B. Zakharov
Leading institutions:
FSUE Special Design Bureau of the Institute of Radiotechnique and electronics of RAS
FSBRI – Institute of Crystallography of RAS
Purpose of the experiment – determination of incubation period for crystal nucleation and growing of
biocrystals by the method of controlled crystallization under conditions of residual microgravity
during flight tests of the BION-M No. 2 SV.
Investigations and experiments on space materials science
1. SE «Study of biologically significant characteristics of space ionizing radiation on board BION-M No. 2
SV”, code «BIORADIATSIYA-2».
Principle Investigator: Candidate of
Physico-mathematical Sciences V. Shurshakov
Leading institute:
Institute of Biomedical Problems of RAS
Purpose of the experiment – study of biologically significant characteristics of space ionizing radiation
and its impacts on bioobjects in open space conditions and inside the biosatellite, and also study and
processing of new methods and means of space dosimetry for their further use in future space missions.
Radiobiological experiments and radiation studies
Scientific hardware
Purpose of the experiment
1. SH «PPN- Bradoz» - assembly of passive detectors
with aluminum collimators
Obtaining of integral (for the whole flight) curves of attenuation of absorbed space
radiation dose over thick protections on the external surface of SV
2. SH «SPD-M» for accommodation of passive
detectors of thermoluminescent and TTD types and of
seeds
Obtaining of integral (for the whole flight) data about absorbed and equivalent doses of
space radiation inside the SV
3. Active dosimeter«RDZ-BZM» (inside SV)
Study of the dynamics of accumulation of absorbed space radiation dose inside SV from
different sources of radiation (galactic space beams, Earth radiation belts, solar proton
events)
4. Active dosimeter of “RDZ-BZM” type (outside the
SV)
Study of the dynamics of accumulation of absorbed space radiation dose outside the SV
over protections from different radiation sources
5. Spectrometer of charged particles “Tritel-B”
Study of the dynamics of accumulation of absorbed and equivalent space radiation doses
inside the SV from different sources of radiation for three protections of different thickness
6. «Bubble-detectors»
Measurement of the contribution into the dose of secondary neutrons.
1. SE «Control of the state and compensation of micro-accelerations on board the BION-M
No. 2 SV by KSKM-2 hardware/software means”, code «KSKM-2».
Principle Investigator : Candidate of Technical Sciences N. Stratilatov
Leading institution:
Space Rocket Center “Progress”
Purpose of the experiment – monitoring of the state of magnetic field inside the descent
module (DM), and also of the field of onboard micro-accelerations, and study of a
possibility of their increasing by means of electromagnets of KSKM-2 hardware during
orbital flight of BION-M No. 2 SV, with the work of System of Moving Control without
provision of controlling impacts.
2. SE «Monitoring of the state of the gas environment parameters, acceleration field, and
kinematic parameters of moving inside the descent module BION-M No. 2 SV”,
code
« MONITOR-SA».
Principle Investigator : Candidate of Technical Sciences N. Stratilatov
Leading institution:
Space Rocket Center “Progress”
Purpose of the experiment - Monitoring of the state of the gas environment parameters,
acceleration field, and kinematic parameters of moving inside the descent module BION-
M No. 2 SV during operations of ground preparation of the SV as a part of carrier rocket,
launch of carrier rocket, and insertion of SV to the orbit, work out of deceleration pulse,
descent of SV to the Earth and landing
.
Physical and technical experiments
3. SE «Study of the impacts of the factors of open space on thin-film instrumental structures”,
code – «CARBON-2».
Principle Investigator: Doctor of Technical Sciences Prof. Yu. Gorelov
Leading institutions:
Samara State University;
FSBRI – Institute of Systems of Image Processing of RAS
Purpose of the experiment – study of the impacts of open space factors on electro-physical
and optical characteristics of engineering models of thin-film instrumental structures, and
also on electro-physical and optical characteristics of engineering models of diffraction
structures based on quartz, chrome film, and resist.
4. SE «Multichannel recorder of temperatures in the containers with scientific hardware on the
external surface of space vehicle” , code – «MRT-2».
Principle Investigator: Doctor of Technical Sciences Prof. Yu. Gorelov
Leading Institution:
Samara State University.
Purpose of the experiment – registration of temperatures of the scientific hardware
elements allocated in the containers with scientific hardware under open space conditions
on the external surface of space vehicle (descent module).
Physical and technical experiments
5*. SE «Study of the prototype of high-stable board source of IR light based on the phase transfer
of eutectic alloy indium – bismuth in space flight conditions”, code «CALIBR-2».
Principle Investigator: Doctor of Technical Sciences Prof. V. Sapritskiy.
Leading institution:
The All-Russian Research Institute for Optical and Physical Measurements Federal State Unitary
Enterprise
Purpose of the experiment –
Study of the phase transfer of melting-crystallization of
eutectic alloy In-Bi with simultaneous testing of the prototype of on-board black body based
on the phase transfer of melting In-Bi as a perspective high-stable source of IR light for on-
board calibration of the hardware for Earth observation under conditions closed to
weightlessness (microgravity).
Physical and technical experiments
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