Sample Preparation Protocol for ACE/Simons Whole Blood Serotonin (5-HT) A whole blood specimen (1.5 – 2.5 mL) is collected in an EDTA-containing tube (lavender stopper) and kept at room temperature. The specimen tube can be kept at room temperature for up to 8 hours* before removing portions for storage. After gentle mixing by 5-6 inversions, a 250 L portion of blood is transferred to a 2 mL plastic screw top storage tube (using a 1 mL Eppendorf pipette & 1000 L tip). A second 250 uL portion is transferred in the same way to a second storage tube in order to prepare a duplicate 250 L sample. Tubes should be labeled with the usual ACE/Simons tube number, and stored in 2 separate boxes.
The blood remaining in the lavender stopper tube should be transferred to a third tube and stored in a separate box. All of the samples are stored at -70 to -80 C and are not thawed until analysis.
* Samples are actually stable for up to 24 hours at room temperature (which is preferred to refrigeration), but the listed times are conservative guidelines which should be adhered to if possible.
The frozen samples are shipped in batch on dry ice to the Yale Child Study Center (call or email to arrange shipping date and confirm address).