Ulla Seppälä, PhD Vaccine Research
What is Allergy ?
Most common sources of inhalant allergens
Allergens and Allergen Diagnostics
What is allergy vaccination ?
Anergy = clonal silence or actual clonal deletion of allergen-reactive T-cells Immunoregulation-induced immune deviation, leading into different cytokine milieu in a target tissue
Targeting the therapeutic tools ?
Therapeutical recombinant proteins / rDNAs
Formulation of allergen vaccines by use of various adjuvants Bacterial origin mucosal adjuvants - CpG ODN,
- Monophosphoryl lipid A (MPL)
- Cholera toxin /CT (Vibrio cholera), entero toxin /LT (Escherichia coli)
Carbohydrates / (CBPs) Microencapsulated allergen vaccines
How to monitor allergen immunotherapy ? - improved clinical phenotype
- serum IgE / IgG4 –levels IgE / IgG4
- decrease in number of mast cells and eosinophils after allergen provocation
- modified and/or reduced production of cytokines
- BIOMARKERS ? !
How to monitor allergen immunotherapy ?
”Genomics and proteomics of allergic disease” Identification of the disease genes; for design of new classes of anti-inflammatory compounds Identification of expression and function of proteins; to obtain increased knowledge of mechanisms underlying allergic disease Identifiction of novel biomarkers
Microarray technology
Proteomics technology
Proteomics in Allergy Research
Biomarker discovery: PEPTIDOMICS Peptides are ideal candidates for biomarkers Proteases liberate biomarkers – processing and specific degradation products – discovery tool ! - Hormones
- Cytokines
- Growth factors
- etc.
Conclusions
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