Bariloche protein symposium argentine society for biochemistry and molecular biology

164
BIOCELL, 27 (Suppl. I), 2003
NT-P3.
APPLICABILITY OF FLUORESCENCE FROM THE
MAILLARD REACTION AS INDICATOR OF
BIOMOLECULE DAMAGE
Matiacevich, Silvia; Santagapita, Patricio and Buera, Pilar.
Dpto. de Industrias. Facultad de Ciencias Exactas y Naturales.
UBA, Buenos Aires, Argentina. E-mail: [e-mail]
The purpose of this work was to analyse the characteristics of
fluorescence development from sugar-carbonyl amine reactions
(Maillard reaction) in foods, biological and model systems, their
manifestations in living organisms, like seeds, and their
relationship to human diseases. In the area of physiology,
fluorescence has continued to be used since 1985, when advanced
glycosylated end-products (AGEs) were detected in human beings
suffering diabetes and other diseases as a consequence of the
reaction of glucose with long-lived proteins. In living organisms,
the excessive non-enzymatic glycosylation of proteins produces
major biological effects, like crosslinking of the modified proteins
and trapping of soluble proteins by glycosylated extracellular
proteins, decreasing proteolysis and promoting human health
deterioration. The characteristic fluorescence of AGEs (ex 370/
em 440 nm) has been used as an indicator of the level of AGE-
modified proteins since 1985 in research work. However, the
chemical structure of many AGE fluorophores is still unknown,
and only some fluorescent products were described. The utility of
fluorescence measurement as index to follow the degree of reaction
was analyzed in systems of variable composition under different
environmental conditions to clarify if fluorescent products can be
considered early markers of the reaction. The conditions under
which chromophores, AGEs and fluorophores are formed are
discussed.
ABSTRACT INDEX
LECTURES
pages
L1 - L10
29 - 31
SYMPOSIA
S1 - S22
32 - 37
ORAL COMMUNICATIONS
Lipids: LI-C1 - LI-C8
37 - 39
Channels and Transporters: CA-C1 - CA-C8
39 - 41
Plants: PL-C1 - PL-C12
41 - 44
Microbiology: MI-C1 - MI-C18
44 - 48
Structural Biology: BE-C1 - BE-C14
48 - 52
Cell Biology: BC-C1 - BC-C14
52 - 55
Signal Transduction: TS-C1 - TS-C8
55 - 57
Biotechnology: BT-C1 - BT-C8
57 - 59
POSTERS
Microbiology: MI-P1 - MI-P95
59 - 83
Biotechnology: BT-P1 - BT-P35
83 - 92
Structural Biology: BE-P1 - BE-P77
 92 - 111
Cell Biology: BC-P1 - BC-P63
111 - 126
Signal Transduction: TS-P1 - TS-P27
127 - 133
Plants: PL-P1 - PL-P56
133 - 147
Lipids: LI-P1 - LI-P37
147 - 156
Channels and Transporters: CA-P1 - CA-P22
157 - 162
Bioenergetics: BG-P1 - BG-P4
162 - 163
New Technologies: NT-P1 - NT-P3
163 - 164

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165
BIOCELL, 27 (Suppl. I), 2003
A
Abate, C.M.
BC-P28; BC-P48
Abdellah, K.B.
MI-P2
Abdian, P.L.
MI-C1; MI-P8
Abramowicz, L.
BT-P16
Acierno, J.P.
BE-P75
Actis-Goretta, L.
BG-P2
Adamo, H.P.
S13; CA-P5; CA-P8
Agostini, E.
S20; BT-P35; PL-P27
Aguero, F.
MI-C6; MI-P2; MI-P83;
BE-P49; BC-P1
Aguilar, O.M.
MI-P51
Aguirre, A.
S18; MI-P3; MI-P14;
Aguirre, R.
BC-P44
Aisen, E.
BC-P50
Alaniz, M.J.T.de
LI-P8
Albar, J.P.
BT-C8
Albertó, E.
MI-P45
Alcalde, M.
BG-P4
Alcaraz, E.
MI-P79
Aleanzi, M.
BE-C14
Alet, A.I.
PL-P50
Alfonso Pecchio, A.R.
BC-P58
Algranati, I.D.
MI-C5
Alito, A.
MI-P31
Alito, A.E.
MI-P38
Alleva, K.
CA-C6
Almaleck, S.H.
LI-P37
Almasia, N.I.
PL-P32
Alonso, G.D.
PL-P38; MI-P1
Alonso, G.L.
CA-C8
Alonso, G.
MI-P24; CA-P2
Alonso, L.
BE-C6; BE-P26; BE-P30
Alonso, M.
CA-P21
Alonso-Romanowski, S.
BT-P13; BT-P18;
LI-P13
Altabe, S.
MI-P6
Altabe, S.G.
LI-P15
Altcheh, J.
MI-P82
Altier, N.
MI-P80
Alvarez, A.
PL-P52
Alvarez, A.F.
MI-P4
Alvarez, C.
BC-C1; MI-P45
Alvarez, F.
MI-P49
Alvarez, H.
MI-P6
Alvarez, H.M.
MI-P4; MI-P10; MI-P73
Alvarez, M.E.
PL-P55
Álvarez, S.
LI-P35
Álvarez, S.M.
TS-P23
Alzari, P.M.
S1
Amadeo, G.
BT-P32
Amadio, A.F.
BT-P19
Ambroggio, E.E.
BE-P21
Amirante, A.
MI-P5
Amodeo, G.
CA-C6
Amongero, M.
MI-P18
Amor, S.
BT-P20
Amoroso, M.J.
MI-P17
Anciart, N.
BT-P12
Anderson, C.M.
PL-P29
Andreo, C.S.
PL-C11; PL-P17;  PL-P37
Andreu, A.B.
PL-P2; PL-P42
Angeletti, S.
LI-P4; LI-P23
Anguiano, O.L.
BC-P49
Ansaldo, M.
BE-P23
Ansay, C.
BE-P23
Antollini, S.S.
BE-P29
Antón, E.A.
BE-P42
Anton, R.
LI-P33
Añón, M.C.
PL-P19; PL-P24; PL-P45; LI-P26
Aphalo, P.
PL-P45
Arabolaza, A.
MI-P6
Aran, M.
NT-P2
Aranda, A.
MI-C7
Aranda, C.
BE-P20
Araya, A.
PL-C8; PL-P10; PL-P33
Arce, D.
PL-P40
Arcuri, B.de
BE-P77
Arcuri, B.F.de
MI-P70
Argañaraz, M.
BC-P57
Argañaraz, M.E.
BC-P48
Argaraña, C.E.
BT-P24; BT-P27; BT-P28;
MI-P37; MI-P74
Argüelles, M.
MI-P43; MI-P65
Argüelles, M.H.
MI-P11
Argüelles, T.
PL-P41
Arias, A.
MI-P80
Armas, P.
BC-P23, BC-P24
Arnau, J.
TS-P14
Arnulphi, C.
LI-P17
Arranz, S.E.
BC-P20
Arregui, C.O.
BC-C10; BC-P39
Arribére, C.
BE-P65
Arribére, M.C.
BE-P34
Asenzo, G.
MI-P7; MI-P28
Assef, Y.A.
CA-C5
Augusto, O.
BG-P4
Aveldaño, M.I.
LI-C4; LI-P31
Avendaño, C.
BC-P50
Avilés, F.X.
BE-P58
B
Baez, D.
LI-P36
Baier, C.J.
BC-C11
Baldi, C.
TS-P18
Balestrasse, K.B.
PL-C10
Ballaré, C.L.
PL-P5
Ballicora, M.
BE-C14
Barbagelata, R.
MI-P71
Barberis, S.
BE-P64; BE-P65; BE-P68;
BE-P69; PL-P48; PL-P49
Barboza, M.
BE-P56
Barchiesi, J.
MI-P29
Barcia, C.
PL-P48; PL-P49
Barneix, A.J.
PL-C4
Baroni, M.V.
PL-P47
Barra, J.L.
MI-P37
Barrantes, F.J.
S6; CA-C7; BC-C11; BE-P29;
LI-P22; CA-P3; CA-P11;
CA-P12; CA-P15; CA-P19
Barreras, M.
MI-C1; MI-P8
Barría, M.E.
MI-P73
Barrientos, E.
BE-P43
Barrio, M.
BC-C7
Bartel, L.C.
BC-P4
Bartos, M.
CA-P20
Baruzzi, A.M.
LI-P3
Basabe, J.C.
LI-C1
Basile, L.
MI-P9
Batista, S.
MI-P63
Bazán, S.
BE-P76
Bazzini, A.A.
PL-P32
Beaugé, L.
CA-C3; CA-P6; CA-P17; CA-P18
Beccaría, A.J.
BT-P23; BT-P34
Begher, S.
MI-P82
Belaich, M.N.
BT-P21; BT-P25
Belizán, A.
MI-P11
Bellini, M.J.
LI-P21
Bello Gay, E.
BE-P57
Bellomio, A.
MI-P70
Belmonte, S.
BC-P26
Beltramino, C.
BC-P11
Beluardi, P.
BE-P50
Benavides, M.P.
PL-C10; PL-P34
Benavidez, P.E.
MI-P50
Bentancor, L.
BT-C6; BT-P3
Berberian, G. CA-C3; CA-P6; CA-P17; CA-P18
Berguer, P.
BT-P12
Berinstein, A.
MI-P81
Bermudez, V.
LI-P22
Bernasconi, A.M.
LI-C1
Berni, J.
BC-P9
Berón, W.
BC-C3; BC-P34
Bianconi, L.
LI-P29
Bianconi, M.L.
BE-P11
Bidinost, C.
MI-C10
Bigi, F.
MI-C9; MI-P31; MI-P38; MI-P68
Bilen, M.F.
BT-P20
Birnbaumer, L.
MI-P24
Biscoglio de Jiménez Bonino, M.       BE-P51
Bisio, M.
MI-P82
Bittel, C.
MI-C2; MI-P20
Blackhall, J.
MI-P43
Blancato, V.S.
MI-C18; MI-P12
Blanco Rivero, M.C.
BE-P6
Blanco, P.
BC-P18
Blatt, M.R.
TS-P26
Blaustein, M.
BC-P13; TS-P13
Boccaccio, G.
BT-P12
Bocco, J.L.
MI-C10; BC-P33
Bolana, R.
TS-P6
Boland, R.
TS-P10; TS-P18; TS-P21; TS-P27
Bollo, M.
TS-P22
Bologna, F.P.
PL-P17
Bonacci, G.
MI-C10; BC-P46
Bonansea, S.
TS-P19; TS-P22
Bondesio, M.E.
MI-P46
Bondino, H.G.
PL-P36
Bonini, I.
LI-P22
Bonomi, H.R.
MI-P36
Bonomi, M.
LI-P34
Bontempi, E.
MI-C4
Borán, M.S.
BE-P1
Borioli, G.
LI-C6
Borioli, G.A.
LI-P14
Bortolotti, A.
MI-C2
Botta, P.E.
MI-C12
Bouvier, L.A.
BE-P12; CA-P4
Bouzat, C.
S16; CA-C2; CA-P7; CA-P13;
CA-P14; CA-P20
Bouzat, C.B.
CA-P10
Boveris, A.
BG-P3
AUTHOR  INDEX
L = Lecture
S = Symposium
C = Communications
P = Posters

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