Guidance Manua pdf



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geosmin.  The presence of certain culprit algae may not necessarily mean that MIB 



and/or geosmin is being produced.  Several of the MIB producers appear to be able to 

turn on/off production in the laboratory.  Production of T&O compounds should be 

verified by GC/MS analysis. 

 

MIB and geosmin. Analyze by FPA, or preferably by GC/MS.  Rapid turnaround is 



paramount for making decisions on water supply management, canal treatment, or in-

plant process options.  Analytical results reported within 72 hours of sampling is a 

reasonable goal. 

 

Flavor Profile Analysis (FPA) panels should be trained by experts and chlorine should be 



quenched with hydrogen peroxide in order to specifically detect MIB or geosmin.   

 

MIB and geosmin can be measured using Solid-Phase Microextraction/Gas 



Chromatography/Mass Spectroscopy (SPME-GC/MS) (Watson et al., 2000; Lloyd et al., 

1998).  Details of the method used in the recent study are given in Appendix C. 

 

4.3  MONITORING IN PRESSURIZED WATER DISTRIBUTION SYSTEMS 

 

Algae do not pass through sand filtration systems at the WTPs nor grow in the absence 



of light, and therefore typically are not present in pressurized pipe water distribution 

systems carrying finished drinking water from the WTPs.  However, fungi, bacteria (e.g., 

actinomycetes) and other organisms can grow in water distribution systems and also 

impart earthy, musty, moldy odors to water.  Therefore, customer complaints about tap 

water may be caused from either: (1) T&O compounds in finished water leaving the WTP 

or (2) T&O compounds produced within the pressurized water distribution system.  If a 

water utility suspects the latter due to numerous complaints from a localized region of the 

water distribution system, then samples from the distribution system should be collected.  

Preferred sampling would include a responsive program where samples from the service 

tap and customer faucet are collected and analyzed for chlorine residual (field) and 

MIB/geosmin (lab).  Many complaints are actually from chlorine residual.  If a routine 

monitoring program is desired, then points within the water distribution system should 

represent points of historic microbial concern (e.g., high plate counts, low chlorine 

residuals). 



4.4  PREDICTION OF T&O PROBLEMS 

4.4.1  Rationale for Prediction 

 

The ability to predict the occurrence of T&O episodes is important for water resources 



planning and for water treatment plant staff for three reasons: 

 

1. 



Some T&O episodes may be prevented.  For example, canal treatments can be 

used to prevent the growth of T&O culprit algae.  To utilize canal treatments 

effectively, it is desirable to know where and when T&O episodes are likely to 



 

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occur, because it is too expensive to treat the entire canal throughout the T&O 



season. 

 

2. 



Some T&O episodes may be avoided.  In many cases, it may not be possible to 

prevent the production of T&O compounds upstream, but water resources 

managers could manage the water supply system to prevent poor-tasting water 

from reaching consumers.  This can be done through reservoir management, 

blending of source waters, or source switching (reducing production at a WTP 

receiving poor-tasting water while simultaneously increasing production at a WTP 

receiving better quality water). These management procedures will be discussed 

later. 


 

3. 


Water treatment plants can remove T&O compounds, but need advance warning to 

do this effectively.  T&O compounds can generally be reduced below threshold 

levels using powdered activated carbon (PAC) within the WTPs.  However, 

treatment plant operators need to know when T&O episodes will occur so that they 

can order PAC and prepare their WTPs for an episode.   

4.4.2  Reservoirs 

 

MIB was produced in all three of the water storage reservoirs above the canal 



distribution system (Section 2).  Prediction of when MIB is likely to be released from each 

reservoir, and its impact on Phoenix’s water supply, is now possible. 

 

As noted above, MIB is produced by certain blue-green algae, which grow primarily 



either in shallow near shore areas or suspended in the epilimnion.  MIB concentrations in 

the epilimnia of all three reservoirs commonly exceed 20 ng/L during the summer 

stratification period.  Concentrations of MIB in the outlets from the reservoirs during 

summer  stratification are nearly always much lower than found in the epilimnion, 

because water is released from the hypolimnion.  Figures 4-2 through 4-4 show MIB 

concentrations in the reservoirs and their outlets.   

 

Lake Pleasant

0

10

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Aug-99

Oct-99

Dec-99

Feb-00

Apr-00

Jun-00

Aug-00

Oct-00

Dec-00

Feb-01

Apr-01

Jun-01

Aug-01

Oct-01

Dec-01

Feb-02

Apr-02

MIB, ng/L

Epilimnion

Hypolimnion

CAP@7th St.

 

Figure 4-2.  MIB in the epilminion, hypolimnion, and outflow from Lake Pleasant. 



 


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