XIV
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International Conference on Molecular Spectroscopy, Białka Tatrzańska 2017
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KEYNOTE LECTURES
K–1 – Michael Heise – Mid-infrared ATR-sensor developments based on silver halide fibers for
spectrometric process analysis and applications in medical sciences and microbiology
K–2 – Małgorzata Barańska – Raman spectroscopy to improve patient diagnosis and
prognosis
K–3 – Rui Fausto – Probing radical and nitrene intermediates in narrowband UV-induced
photoreactions in cryogenic inert matrices
K–4 – Keith Gordon – Generating long-lived charge-separated excited states from blended
organic, inorganic chromophore systems: a spectroscopic and computational study
K–5 – Maurizio Ferrari – Glasses and glass-ceramics for photonics: advances and
perspectives
K–6 – Stefan Jurga – Block copolymers in nanoscience: microscopy and spectroscopy
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International Conference on Molecular Spectroscopy, Białka Tatrzańska 2017
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K–1
Mid-infrared ATR-sensor developments based on silver halide fibers
for spectrometric process analysis and applications in medical sciences
and microbiology
H. Michael Heise
1
, and Lukas Küpper
2
1
Interdisciplinary Center for Life Sciences, South-Westphalia University of Applied Sciences,
Frauenstuhlweg 31, D-58644 Iserlohn, Germany, e-mail: heise.h@fh-swf.de
2
Infrared fiber sensors, Im Gillesbachtal 33, D-52066 Aachen, Germany
In recent years, infrared spectroscopy has seen much progress in instrumentation and
measurement techniques [1–3]. It has been used for monitoring in many processes of the
chemical and pharmaceutical industry, in addition to the broad field of food industrial and
biotechnological applications. Therefore, Process Analytical Technology (PAT) has been in the
focus of the chemical industry, as many stages in the industrial production exist where analytical
information is needed. Samples, for example, entering the factory premises can be liquids or
powders, and their identification or their composition can be checked by fast infrared
spectroscopic methods without additional expenditure, but often continuous monitoring in many
synthesis processes has been utilized in combination with chemometric tools such as
multivariate curve resolution (MCR).
Easy sample handling including remote sensing capability can be provided by fiber-optic
probes fabricated from infrared transparent polycrystalline silver halide fibers. Optical
characteristics of such fibers are presented that are important for sensor design, but also
degradation is discussed. Advances in the quality of silver halide fibers and their extrusion with
different cross-sections enabled us to construct several inert sensor probe heads, mainly with the
coupling of a diamond micro-prism, suited for process monitoring and quasi-continuous
measurements. Transmission and attenuated total reflection techniques have been mainly
employed for the analysis of liquids with the latter favored by us, while solids and powders can
be best analyzed by reflection spectroscopy. Other recent applications are in medical sciences
with examples given from dermatology, oncology and clinical chemistry. Microbiology is
another area, where fiber-coupled diamond probes were used for characterization of micro-
algae, bacteria and fungal mycelium in combination with fast micro-techniques. Several
applications with such fiber-optic sensor probes are presented.
Keywords: silver halide fibers; infrared ATR-spectroscopy; process analytical technology;
life science applications
References
[1] H.M. Heise, J. Fritzsche, H. Tkatsch, F. Waag, K. Karch, K. Henze, S. Delbeck, J. Budde, Eur. J.
Phys. 34 (2013) S139–S159
[2] H.M. Heise, Biomedical Vibrational Spectroscopy–Technical Advances, [in:] Biomedical Vibrational
Spectroscopy
, P. Lasch and J. Kneipp (Eds.), Chapter 2, 9–37, Wiley (2008).
[3] H.M. Heise, S. Delbeck, L. Küpper, Recent advances in sensor developments based on silver halide
fibers for mid-infrared spectrometric analysis
, [in:] Molecular and Laser Spectroscopy: Advances and
Applications
, V.P. Gupta (Ed.), Chapter 3, Elsevier, San Diego (CA) (2017) in press.
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K–2
Raman spectroscopy to improve patient diagnosis and prognosis
Małgorzata Barańska
1,2
1
Faculty of Chemistry, Jagiellonian University, Krakow, Poland,
2
Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Krakow,
Poland, e-mail: baranska@chemia.uj.edu.pl
There have been significant advances in vibrational biomedical spectroscopy in recent
years. Diagnostic and prognostic tools based on the new technologies have the potential to
revolutionize our clinical systems leading to improved patient outcome, more efficient
public services and significant economic savings for healthcare society.
In particular, the advantages as well as limitations of Raman spectroscopy in biomedical
samples analysis are presented. Several examples of application of confocal Raman
imaging to study in vitro endothelial cells, and ex vivo tissue of various mice models (e.g.
atherosclerosis, diabetes, fatty liver) is demonstrated, including a novel approach to
investigate primary cells isolated from the liver and heart. A general overview on other
Raman spectroscopy techniques, e.g. Coherent anti-Stokes Raman Spectroscopy (CARS),
Surface Enhanced Raman Spectroscopy (SERS), Tip Enhanced Raman Spectroscopy
(TERS), Stimulated Raman Spectroscopy (SRS), in the medical samples studies is also
shown. Perspectives of the translation of Raman spectroscopy into the clinical environment
for the general benefit of patients are discussed.
Keywords: Raman spectroscopy; imaging; CARS; SERS; TERS; SRS
Acknowledgements
This
work
was
supported
by
National
Science
Center
(DEC-2015/16/W/NZ4/00070
and DEC-2016/22/M/ST4/00150).
References
[1] J. Dybas, K. M. Marzec, M. Z. Pacia, K. Kochan, K. Czamara, K. Chrabaszcz, E. Staniszewska-
Slezak, K. Malek, M. Baranska, A. Kaczor, Trends Anal. Chem. 85 (2016) 117.
[2] M. Baranska (ed.), Optical Spectroscopy and Computational Methods in Biology and Medicine,
Springer, Series: Challenges and Advances in Computational Chemistry and Physics, Vol. 14 (2014),
ISBN 978-94-007-7831-3.
[3] H.J. Byrne, M. Baranska, G.J. Puppels, N. Stone, B. Wood, K.M. Gough, P. Lasch, P. Heraud, J. Sulé-
Suso, G.D. Sockalingum, Analyst 140 (2015) 2066.
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