XIV
h
International Conference on Molecular Spectroscopy, Białka Tatrzańska 2017
92
T3: O–11
Adsorption geometry variations of the anticancer drug
onto potential colloidal nanocarriers
Natalia Piergies
1
, Czesława Paluszkiewicz
1
, Magdalena Oćwieja
2
,
and Wojciech M. Kwiatek
1
1
Institute of Nuclear Physics Polish Academy of Sciences, PL-31342 Krakow, Poland,
e-mail: natalia.piergies@ifj.edu.pl
2
J. Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences, PL-30239
Krakow, Poland
The epidermal growth factor receptor (EGFR) inhibitor, erlotinib is used in the target
therapy of the non-small cell lung cancer (NSCLC). However, the low specificity of this drug
causes many side effects such as skin rash and diarrhoea [1]. It has been proven, that the
nanoparticles have higher affinity to the tumor cell and more readily accumulate inside them [2].
Thus, nanoparticles may be used as an effective drug delivery system which could reduce the
side effects.
In this study, the surface-enhanced Raman spectroscopy (SERS) was applied to characterize
the adsorption geometry the investigated drug immobilized onto colloidal Au and Ag
nanoparticles in various conditions (e.g. time of incubation). The Fig. 1 presents the SERS
spectra collected upon different incubation time at 37°C. The observed spectral pattern indicates
that the erlotinib adsorption geometry onto the both SERS – active nanoparticle systems changes
as the incubation time increases.
37
O
C/ time dependent
Au coll
Ag coll
0.5 h
24 h
48 h
24 h
48 h
72 h
R
am
an
in
te
ns
ity
R
am
an
in
te
ns
ity
2000 1600 1200 800 400
2000 1600 1200 800 400
wavenumbers/cm
-1
wavenumbers/cm
-1
Fig. 1. The SERS spectra of erlotinib immobilized onto the Au and Ag colloidal nanoparticles
at 37°C after 0.5, 24, 48 and 24, 48, 72 hours adsorption, respectively.
Keywords: erlotinib; surface-enhanced Raman spectroscopy (SERS); Au nanoparticles; Ag nanoparticles
Acknowledgment
This project has been supported by the National Science Centre Poland under decision no UMO-
2016/21/D/ST4/02178. This research was performed using equipment purchased in the frame of the project
co-funded by the Małopolska Regional Operational Program Measure 5.1 Krakow Metropolitan Area as an
important hub of the European Research Area for 2007-2013, project No. MRPO.05.01.00-12-013/15.
References
[1] C. Gridelli, P. Maione, M.N. Bareschino, C. Schettino, P.C. Sacco, R. Ambrosio, V. Barbato, M.
Falanga, A. Rossi, Anticancer Res. 30 (2010) 1301.
[2] Z.P. Xu, Q.H. Zeng, G.Q. Lu, A.B. Yu, Chem. Eng. Sci. 61 (2006) 1027.
XIV
h
International Conference on Molecular Spectroscopy, Białka Tatrzańska 2017
93
T3: O–12
Monitoring the interfacial behavior of Lu AA33810 after conjugation
with gold nanoparticles: SERS and SEIRA studies
Ewa Pięta
1
, Natalia Piergies
1
, Magdalena Oćwieja
2
, Helena Domin
3
,
Czesława Paluszkiewicz
1
, Elżbieta Bielańska
2
, and Wojciech M. Kwiatek
1
1
Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland,
e-mail: Ewa.Pieta@ifj.edu.pl
2
J. Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences, PL-30239
Krakow, Poland
3
Institute of Pharmacology, Polish Academy of Sciences, Department of Neurobiology, 31-343
Krakow, Smetna Street 12, Poland
Optical properties of molecules immobilized onto roughened metallic substrate may change
significantly [1]. Surface-enhanced Raman spectroscopy (SERS) is a great tool that combines
the specificity of conventional vibrational spectroscopy with ultra high sensitivity at the
molecular level. A similar effect can be achieved in the mid infrared region, where the signal
can be enhanced even three orders of magnitude in regard to the conventional infrared spectrum
[2]. This method was named surface-enhanced infrared absorption spectroscopy (SEIRA).
This work reports the first detailed characterization of molecular structure of Lu AA33810, a
selective Y5 receptor antagonist, and its behavior at the solid/liquid interface after conjugation
with gold nanoparticles (GNPs).
GNPs
Lu AA33810
a
b
c
Fig. 1. Typical SEM micrographs presenting GNPs (a) and LuAA 33810 – GNPs system (b) together with the
suggested adsorption geometry of Lu AA33810 onto studied GNPs [unpublished data].
The performed analysis revealed the usefulness of simultaneous use of both surface-
enhanced vibrational spectroscopic techniques, SERS and SEIRA, which ensures better
monitoring of the adsorption phenomenon.
Keywords: SERS; SEIRA; gold nanoparticles
Acknowledgment
The research was performed by the use of the equipment purchased in the frame of the project co–funded
by the Małopolska Regional Operational Program Measure 5.1 Krakow Metropolitan Area as an important
hub of the European Research Area for 2007–2013, project No. MRPO.05.01.00–12–013/15.
References
[1] K. Kneipp, G. Hinzmann, D. Fassler, Chem. Phys. Lett. 99 (1983) 5.
[2] M. Osawa, Top. Appl. Phys. 81 (2001) 163.
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