Appendix 2-5: Rejected ecotox bibliography Excluded

Keywords: Age
Keywords: Farms
Keywords: Housing
Keywords: Organophosphates
Keywords: Risk Abstracts; Health & Safety Science Abstracts
Keywords: Brain
Keywords: H 1000:Occupational Safety and Health
Keywords: USA, Wisconsin
Keywords: Arsenates
Keywords: USA, Minnesota
Keywords: Cancer
Keywords: Environmental Studies
Keywords: USA, Iowa
Keywords: Pesticides
Keywords: R2 23060:Medical and environmental health
Keywords: Glioma English. Date revised - 2013-03-01. Last updated - 2013-03-21. DOI - OB-51c03174-a0fb-43d3-b006mfgefd107; 17720860; 1476-069X. SubjectsTermNotLitGenreText - Age; Farms; Housing; Organophosphates; Pesticides; Brain; Glioma; Arsenates; Cancer; USA, Iowa; USA, Wisconsin; USA, Minnesota

705. Yousif, I. O. Micronucleus analysis and mitotic index in a Jordanian population exposed to pesticides of organophosphate: malathion and chlorpyrifos. 2011; 64, 173-178.

Rec #: 16630
Keywords: HUMAN HEALTH
Notes: Chemical of Concern: MLN
Abstract: Abstract: A longitudinal study of possible genetic damage in Jordanian workers occupationally exposed to a mixture of pesticides containing malathion and chlorpyrifos was studied. Micronucleus assay and mitotic index were selected to achieve this purpose. A second series of blood samples was taken 8 months after the workers were removed from agricultural field to detect DNA repair in lymphocytes of the same subjects. Regardless of the sampling time the exposed workers showed an increased number of micronucleus (MN) frequency. After 8 months of non-exposure the workers showed a significantly decreased number of MN frequency compared to the results of the first sampling, but it was still significantly higher than the controls indicating a possible risk of cytogenetic damage for the exposed group. Besides that, malathion and chlorpyrifos lowered the mitotic index in peripheral lymphocyte cells of the exposed subjects.
Number of Volumes: 2
ISI Document Delivery No.: 844KW ://CCC:000296747100006

706. Yu, Hongbin ; Wang, Xinhong; Sun, Hongwei, and Huo, Mingxin. Photocatalytic degradation of malathion in aqueous solution using an AuÇôPdÇôTiO2 nanotube film. 2010 Dec 15-; 184, (1Çô3): 753-758.

Rec #: 60
Keywords: FATE
Notes: Chemical of Concern: MLN
Abstract: The extensive use of pesticides has promoted the agricultural production, but a series of subsequent environmental issues have drawn the concern of governments and people worldwide, such as groundwater and surface water pollutions. In order to remove these pollutants, photocatalysis has emerged as a powerful method. In this paper, the photocatalytic degradation of an organophosphorus pesticide malathion was investigated using an AuÇôPd co-modified TiO2 nanotube film (AuÇôPdÇôTiO2). This film was fabricated by simultaneously photo-depositing Au and Pd precursors on a self-organized TiO2 nanotube film. Its morphology and structures were well characterized by a scanning electron microscope (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The photocatalytic experiments revealed that the malathion elimination rate increased by 172% when the photocatalyst of the naked TiO2 nanotube film was replaced by AuÇôPdÇôTiO2. Additionally, the amount of H2O2 yielded on the AuÇôPdÇôTiO2 film in 60 min was 2.89 times that on the naked TiO2. The enhanced photocatalytic performance could be attributed to both the effective separation of photo-generated charge carriers and the higher synthesis rate of H2O2. The possible photocatalytic mechanism was discussed. Photocatalytic degradation/ Organophosphorus pesticide/ Malathion/ AuÇôPdÇôTiO2 http://www.sciencedirect.com/science/article/pii/S0304389410011192

707. Yu, Hongbin ; Wang, Xinhong; Sun, Hongwei; Huo, Mingxin, and Yu, Hongbin. Photocatalytic Degradation of Malathion in Aqueous Solution Using an Au-Pd-Tio2 Nanotube Film. 2010 Dec 15; 184, (1-3): 753-758.

Rec #: 3830
Keywords: FATE
Notes: Chemical of Concern: MLN
Abstract: Abstract: The extensive use of pesticides has promoted the agricultural production, but a series of subsequent environmental issues have drawn the concern of governments and people worldwide, such as groundwater and surface water pollutions. In order to remove these pollutants, photocatalysis has emerged as a powerful method. In this paper, the photocatalytic degradation of an organophosphorus pesticide malathion was investigated using an Au-Pd co-modified TiO2 nanotube film (Au-Pd-TiO2). This film was fabricated by simultaneously photo-depositing Au and Pd precursors on a self-organized TiO2 nanotube film. Its morphology and structures were well characterized by a scanning electron microscope (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The photocatalytic experiments revealed that the malathion elimination rate increased by 172% when the photocatalyst of the naked TiO2 nanotube film was replaced by Au-Pd-TiO2. Additionally, the amount of H2O2 yielded on the Au-Pd-TiO2 film in 60min was 2.89 times that on the naked TiO2. The enhanced photocatalytic performance could be attributed to both the effective separation of photo-generated charge carriers and the higher synthesis rate of H2O2. The possible photocatalytic mechanism was discussed.
Keywords: Scanning electron microscopy
Keywords: Pesticides (organophosphorus)
Keywords: Surface water
Keywords: Agricultural production
Keywords: Spectroscopy
Keywords: X-ray diffraction
Keywords: Malathion
Keywords: ENA 06:Food & Drugs
Keywords: Pollutants
Keywords: Photodegradation
Keywords: Toxicology Abstracts; Environment Abstracts
Keywords: Hydrogen peroxide
Keywords: Ionizing radiation
Keywords: Pesticides
Keywords: Ground water
Keywords: Groundwater
Keywords: X 24330:Agrochemicals
Keywords: Photoelectron spectroscopy
Keywords: nanotechnology English. Date revised - 2011-01-01. Last updated - 2012-03-29. DOI - 94e42622-9244-41f8-8771csamfg201; 13888660; 0304-3894. SubjectsTermNotLitGenreText - Pesticides (organophosphorus); Scanning electron microscopy; Pollutants; Surface water; Hydrogen peroxide; Ionizing radiation; Ground water; X-ray diffraction; Photoelectron spectroscopy; Malathion; Photodegradation; Agricultural production; Pesticides; Spectroscopy; Groundwater; nanotechnology

708. Yu, W. W. and White, I. M. Inkjet-printed paper-based SERS dipsticks and swabs for trace chemical detection. 2013; 138, 1020-1025.

Rec #: 16660
Keywords: CHEM METHODS
Notes: Chemical of Concern: MLN
Abstract: Abstract: We demonstrate a paper-based surface swab and lateral-flow dipstick that includes an inkjet-printed surface-enhanced Raman spectroscopy (SERS) substrate for analyte detection. Due to capillary-action wicking of cellulose, the paper dipstick enables extremely simple and pump-free loading of liquid samples into the detection device, and in addition provides inherent analyte concentration within the detection volume. Furthermore, the flexible nature of the paper-based SERS device also enables it to act as a swab to collect analyte molecules directly from a large-area surface; the collected analyte molecules can then be focused into a small-volume SERS-active region by lateral-flow concentration. These capabilities are unseen in today's SERS substrates and microfluidic SERS devices. Using these novel lateral-flow paper SERS devices, we achieved detection limits as low as 95 fg of Rhodamine 6G (R6G), 413 pg of the organophosphate malathion, 9 ng of heroin, and 15 ng of cocaine. Moreover, the measurements show that the technique is quantitative and is repeatable across multiple swabs and dipsticks. The results reported here may lead to ultra-low-cost portable applications in trace chemical detection.
Number of Volumes: 4
ISI Document Delivery No.: 074ID ://CCC:000313805200009

709. Yu, W W; White, I M, and Yu, W W. A Simple Filter-Based Approach to Surface Enhanced Raman Spectroscopy for Trace Chemical Detection. 2012 Mar 7; 137, (5): 1168-1173.

Rec #: 2740
Keywords: CHEM METHODS
Notes: Chemical of Concern: MLN
Abstract: Abstract: We demonstrate an extremely simple and practical surface enhanced Raman spectroscopy (SERS) technique for trace chemical detection. Filter membranes first trap silver nanoparticles to form a SERS-active substrate and then concentrate analytes from a mL-scale sample into a mu L-scale detection volume. We demonstrate a significant improvement in detection limit as compared to colloidal SERS for the pesticide malathion and the food contaminant melamine. The measured SERS intensity exhibits low variation relative to traditional SERS techniques, and the data can be closely fit with a Langmuir isotherm. Thus, due to the simple procedure, the low-cost of the substrates, the quantitative results, and the performance improvement due to analyte concentration, our technique enables SERS to be practical for a broad range of analytical applications, including field-based detection of toxins in large-volume samples.
Keywords: British Isles
Keywords: Filters
Keywords: Membranes
Keywords: P 9999:GENERAL POLLUTION
Keywords: Pesticides
Keywords: Pollution Abstracts; Water Resources Abstracts
Keywords: Spectroscopy
Keywords: Silver
Keywords: Toxins
Keywords: Malathion English. Date revised - 2012-04-01. Last updated - 2012-12-14. DOI - MD-0018433158; 16506799; 0003-2654. SubjectsTermNotLitGenreText - Filters; Membranes; Pesticides; Spectroscopy; Silver; Malathion; Toxins; British Isles

710. Yu, Y.; Yang, A. M.; Zhang, J. H.; Hu, S. K., and Yan, H. [Synergistic Effect of Dichlorvos, Dimethoate and Malathion Mixture on Reproduction Toxicity in Male Mice].

Rec #: 8450
Keywords: NON-ENGLISH
Notes: Chemical of Concern: MLN
Abstract: ABSTRACT: OBJECTIVE: To evaluate the reproduction toxicity of the mixture composed of dichlorvos, dimethoate and malathion synergistic effect on male mice, and further explore its possible mechanisms.
ABSTRACT: METHODS: The 105 male mice were divided into 7 groups, including control (0 mg/kg), mix low (10.8 mg/kg), mix medium (21.5 mg/kg), mix high dose (43.0 mg/kg), dichlorvos (5.1 mg/kg), dimethoate (12.6 mg/kg) and malathion (25.3 mg/kg) group. The oral gavage for successive 35 days, and the mice were sacrificed on the 36(th) day. The body weight, and the quantity, activity and morphology of sperms were examined. The levels of sexual hormone were measured, including testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E(2)). Pathological changes of testicle and epididymis were observed by morphology, pathology and electron microscope.
ABSTRACT: RESULTS: After 14 days exposure, the body weights of the mice were lower in the mix-high dose group ((22.40 ± 3.07) g) than those in control group ((26.73 ± 2.82) g) (P < 0.05). After 28 days exposure, the body weights of the mice were also lower in the mix-medium dose group ((30.00 ± 4.93) g) than those in control group ((33.13 ± 3.29) g) (P < 0.05). The sperm counts and sperm motility decreased significantly as the toxic concentration arised. Comparing to control group ((373.33 ± 14.65)×10(6)/g weight of epididymis and (75.17 ± 7.68)%), the spermatozoa count and sperm motility had decreased in mix-medium and mix-high dose groups ((321.17 ± 18.19)×10(6)/g weight of epididymis, (225.00 ± 19.67)×10(6)/g weight of epididymis, and (64.67 ± 9.91)%, (57.83 ± 9.66)%), and the sperm abnormality rates were higher in mix-medium and mix-high groups ((43.33 ± 8.66)‰ and (55.00 ± 13.80)‰) comparing to those in control group ((32.67 ± 8.17)‰). Compared to those in control group (FSH (1.41 ± 0.20), E(2)(17.32 ± 2.72), LH (8.75 ± 1.32) and T (3.45 ± 0.80) nmol/L), the serum level of FSH (3.14 ± 0.62) and (3.85 ± 0.37) nmol/L, E(2) (36.81 ± 6.68) and (43.76 ± 9.82) nmol/L in mix-medium and mix-high dose group increased (P < 0.01), while the level of LH (5.21 ± 1.23) and (4.27 ± 1.09) nmol/L and T (1.37 ± 0.38) and (0.73 ± 0.18) nmol/L decreased (P < 0.01). The morphological and ultramicrostructure results of testicle and epididymis indicated that the mature sperm numbers were decreased, and the cacoplastic sperm head and the tail of spermatozoon were observed in mix-high dose groups.
ABSTRACT: CONCLUSION: The dichlorvos, dimethoate and malathion mixture had synergistic reproductive toxicity to the testicle and epididymis structure and function, and thus leading to the process of generation cell cytopoiesis abnormalities, simultaneously the hypothalamus-pituitary-gonad axis were also affected and thus resulted in parasecretion.
MESH HEADINGS: Animals
MESH HEADINGS: Body Weight
MESH HEADINGS: Dichlorvos/*toxicity
MESH HEADINGS: Dimethoate/*toxicity
MESH HEADINGS: Malathion/*toxicity
MESH HEADINGS: Male
MESH HEADINGS: Mice
MESH HEADINGS: Mice, Inbred ICR
MESH HEADINGS: Organ Size
MESH HEADINGS: Sperm Count
MESH HEADINGS: Sperm Motility
MESH HEADINGS: Spermatozoa/*drug effects
MESH HEADINGS: Toxicity Tests chi

711. Yuan, C. Y.; Cui, Q. M., and Shao, Q. The Effects of Four Kinds of Insecticides on Growth of Green Alga Scenedesmus obliquus. Tianjin University of Science & Technology, Tianjin 300457,China//: 2009; 28, (9): 525-527(CHI) (ENG ABS).

Rec #: 1910
Keywords: NON-ENGLISH
Call Number: NON-ENGLISH (CYP,MLN)
Notes: Chemical of Concern: CYP,MLN

712. Yue, Y. X.; Li, L.; Liang, Y.; Dong, L. J.; Dong, Q. J., and Zhong, H. Y. Effects of co-existed proteins on measurement of pesticide residues in blood by gas chromatography-mass spectrometry. 2010; 878, 3089-3094.

Rec #: 16670
Keywords: CHEM METHODS
Notes: Chemical of Concern: MLN
Abstract: Abstract: Accurate measurement of pesticides in biological fluids such as blood is important for quantifying environmental exposures. Beyond sample enrichment and separation, the method presented here is focused on studies of interactions between pesticides and co-existed proteins. It was experimentally demonstrated that entrapped or adsorbed pesticide residues within the folded native structures of proteins were poorly recovered using direct solvent extraction solely. We described here an effective approach termed Enzymatic Digestion-Organic Solvent Extraction (eDOSE) that utilizes the enzymatic approach to disrupt the folded structures of proteins and release entrapped or adsorbed pesticide residues. In this approach. samples were first reduced, alkylated, tryptically digested and then diluted 10 times before the subsequent extraction using an n-hexane solution. Resultant pesticide residues were determined by capillary gas chromatography coupled with a mass spectrometer. Mean recoveries of the 5 organophosphorus pesticides pre-spiked in fish blood including diazinon, parathion-methyl, malathion, parathion-ethyl and ethion were 85%, 95%, 84%, 103%, and 43% respectively using eDOSE strategy but only 24%, 45%, 40%, 27%, and 29% respectively using direct solvent extraction approach. The eDOSE approach was effective for demonstrating the critical role of folded native structure of serum albumin in adsorption of exogenous chemicals. It provides an alterative means for denaturation of proteins when the target analytes are not stable in acidic solution or entrapped within the protein aggregates caused by organic solvents such as acetone that have been applied for protein denaturation. The eDOSE approach should be able to combine with other advanced techniques of enrichment and separation for more efficient and accurate measurement of target compounds present in the context of complex biological systems. This approach can provide wide applications to the analysis of a variety of small molecules including environmental pesticide residues and metabolites as well as other toxins present in cells, tissues and biofluids. (C) 2010 Elsevier B.V. All rights reserved.
Number of Volumes: 30
ISI Document Delivery No.: 686BR ://CCC:000284672300007

713. Zaja, Roko; Loncar, Jovica; Popovic, Marta; Smital, Tvrtko, and Zaja, Roko. First Characterization of Fish P-Glycoprotein (Abcb1) Substrate Specificity Using Determinations of Its Atpase Activity and Calcein-Am Assay With Plhc-1/Dox Cell Line. 2011 May; 103, (1-2): 53-62.

Rec #: 6380
Keywords: IN VITRO
Notes: Chemical of Concern: MLN
Abstract: Abstract: P-glycoprotein (P-gp; abcb1) is one of the major ABC transport proteins that mediates multixenobiotic resistance (MXR) defense in fish. In order to offer a sound evaluation of its ecotoxicological relevance it is critical to characterize substrate specificity of fish P-gp. Measurement of the ATPase activity is a reliable approach often used to discern type of interaction of various drugs with mammalian P-gp. A similar assay has never been used for characterization of P-gp in aquatic organisms and the main goal of this study was to develop a specific ATPase assay for characterization of fish P-gp. For this purpose we have used P-gp enriched membrane vesicles isolated from fish hepatoma PLHC-1/dox cells characterized by high overexpression of P-gp. As additional demonstration of a P-gp specific phenotype, we have quantified transcript expression of a series of eight ABC efflux transporter genes constitutively expressed in PLHC-1 wild type and PLHC-1/dox cells. Transcript expression analysis confirmed high and specific P-gp transcript overexpression in PLHC-1/dox cells. Provided that the transcript abundance is translated to protein, the development of ATPase assay is enabled. Using this model we determined KmATP of 0.4mM, baseline ATPase activity from 35-50nmol/mgPROT/min, and maximal activation of ATPase activity obtained for fish P-gp in our system was 1.8-2.5-fold over baseline. All these values were in good agreement with data previously reported for mammalian P-gp. In order to perform a more detailed characterization of fish P-gp substrate specificity, in the next step of our study we used the developed ATPase assay to test 50 different compounds for their interaction with fish P-gp. The same set of compounds was also tested with calcein-AM (Ca-AM) transport activity assay both using PLHC-1/dox cells and NIH 3T3/MDR1 fibroblast cells overexpressing human P-gp. Our results showed that there is a clear difference for some substancesafive compounds specifically interacted only with fish P-gp, while seven compounds exhibited interaction with human P-gp only. Most of the compounds tested in this study showed similar behavior in respect to fish or human P-gp and relatively high correlation in the interaction potency was found between fish and human P-gp. In summary, the described results represent the first in depth insight into substrate specificity of an important xenobiotic efflux transporter in fish. In addition, our study showed that combination of Ca-AM assay and the developed ATPase assay using inside/out vesicles isolated from PLHC-1/dox cells, offers a high-throughput and reliable approach for identification of environmentally relevant pollutants that interact with fish P-gp.
Keywords: Q5 01503:Characteristics, behavior and fate
Keywords: Aquatic organisms
Keywords: Specificity
Keywords: Man-induced effects
Keywords: Substrate specificity
Keywords: Xenobiotics
Keywords: Phenotypes
Keywords: Fibroblasts
Keywords: Hepatoma
Keywords: P-Glycoprotein
Keywords: Pollutants
Keywords: Interspecific relationships
Keywords: Membrane vesicles
Keywords: Vesicles
Keywords: Drugs
Keywords: X 24300:Methods
Keywords: Toxicology
Keywords: Drug interaction
Keywords: Membranes
Keywords: Adenosinetriphosphatase
Keywords: Data processing
Keywords: P 2000:FRESHWATER POLLUTION
Keywords: Environment Abstracts; Toxicology Abstracts; Pollution Abstracts; ASFA 3: Aquatic Pollution & Environmental Quality
Keywords: Environmental Studies--Toxicology And Environmental Safety
Keywords: Assays
Keywords: Transcription
Keywords: ENA 02:Toxicology & Environmental Safety
Keywords: Proteins
Keywords: Fish
Keywords: abundance English. Date revised - 2011-10-01. Last updated - 2011-12-08. DOI - OB-74dcae3b-d4e9-40e4-9dadcsaobj201; 14878605; CS1148326; 0166-445X. SubjectsTermNotLitGenreText - Pollutants; Interspecific relationships; Specificity; Man-induced effects; Phenotypes; Drugs; Toxicology; Drug interaction; Hepatoma; Aquatic organisms; P-Glycoprotein; Data processing; Adenosinetriphosphatase; Membrane vesicles; Transcription; Substrate specificity; Vesicles; Fibroblasts; Membranes; Assays; Proteins; Fish; Xenobiotics; abundance

714. Zakeri, H; Bueckert, R a; Schoenau, J J; Vandenberg, a; Lafond, G P, and Zakeri, H. Controlling Indeterminacy in Short Season Lentil by Cultivar Choice and Nitrogen Management. 2012 May 13; 131, 1-8.

Rec #: 2660
Keywords: NO TOXICANT
Notes: Chemical of Concern: MLN
Abstract: Abstract: Western Canada is a major area of lentil (Lens culinaris Medik.) production and global export, specializing in short season production. In cool or wet years lentil often fails to mature within the 115-d growing season. In the absence of a determinate cultivar, our goal was to test whether current cultivars could mature earlier and without a yield penalty by managing N fertility practices. Eight cultivars of lentil were grown in Saskatchewan during 2006 and 2007 under three N fertility treatments: 50 kg N ha-1, Rhizobium inoculant, and an uninoculated control. Measurements included days to maturity, yield, aboveground biomass (DW), plant total N content, and N2 fixation. Lentil yielded 1900 kg ha-1, 2120 kg ha-1 and 2070 kg ha-1 in control, fertilized, and inoculated treatments, respectively. The N treatments did not affect days to maturity or harvest index (HI). Yield and N2 fixation were unaffected by the N treatments except in drought, where yield was smallest in the control and N2 fixation was smallest in the fertilized treatment. Cultivars CDC Milestone, CDC Red Rider and CDC Rouleau had comparable yield and matured earlier than large-seeded cultivars, demonstrating that growing a recently released small-seeded cultivar gave earlier maturity than N fertility management. Results did not demonstrate any advantage from applying N fertilizer to hasten maturity, to increase HI or yield compared to the current practice of relying on N2 fixation from proper inoculation. Biological N2 fixation is a sustainable and more economical means of supplying N to the lentil crop without the need to apply N fertilizer, particularly with high yielding early maturing cultivars.
Keywords: Sustainability Science Abstracts; Environment Abstracts
Keywords: Fertility
Keywords: exports
Keywords: maturity
Keywords: Canada, Saskatchewan
Keywords: M3 1010:Issues in Sustainable Development
Keywords: Lens culinaris
Keywords: Agrochemicals
Keywords: Crops
Keywords: ENA 06:Food & Drugs
Keywords: Fertilizers
Keywords: Rhizobium
Keywords: Economics
Keywords: Cultivars
Keywords: Nitrogen English. Date revised - 2012-05-01. Last updated - 2012-06-29. DOI - 60b23baf-8bcd-4dea-8296csamfg201; 16604687; 0378-4290. SubjectsTermNotLitGenreText - exports; Fertility; Fertilizers; maturity; Economics; Cultivars; Agrochemicals; Crops; Nitrogen; Rhizobium; Lens culinaris; Canada, Saskatchewan

715. Zayats, M. F.; Leschev, S. M.; Petrashkevich, N. V.; Zayats, M. A.; Kadenczki, L.; Szit+ís, R.; Dobrik, H. Szem+ín, and Kereszt+_ny, N. Distribution of pesticides in n-hexane/water and n-hexane/acetonitrile systems and estimation of possibilities of their extraction isolation and preconcentration from various matrices. 2013 Apr 24-; 774, (0): 33-43.